Context
Microbes such as yeast or bacteria are difficult to lyse due to their thick cell wall, which makes nucleic acid extraction difficult. Gram positive bacteria for example, have a cell envelope comprised of the plasma membrane and a thick cell wall containing peptidoglycans. The most common technique for microbial extraction is the use of chemical lysis reagents such as ionic detergent followed by physical grinding with mortar and pestle in liquid nitrogen. The Precellys® homogenizers offer an alternative and more effective lysing solution for DNA and RNA extraction to be used for molecular biology research, such as sequencing, PCR or cloning.
With the new 15 ml tube designed for micro-organisms lysing, you can reach a higher volume (up to 9 ml) of lysed suspension at once.
