[Application note] Flow cytometry analysis of PrPC Knockdown by siRNA in hDPSCs

Stem cells can develop into multiple cell types through a process called differentiation. The cellular form of prion protein (PrPC) has been reported to be involved in various cellular pathways and could also have a role in the differentiation of stem cells into neurons.

Several studies have shown that the prion protein PrPC may be subjected to post-translational cleavage events, producing active fragments such as N1, N2, or shed PrPC that seems to be implied in signalization processes. Here, we examine the impact of shed PrPC in signal pathways and the neuronal differentiation process of stem cells using human dental pulp stem cells (hDPSCs) as a cellular model system.

In this application note, discover how the impact of prion proteins on differentiation and signal pathways was analyzed with flow cytometry using mouse anti-PrP SAF32 mAb from Bertin Bioreagent.

Bertin Bioreagent offers several high-quality prion antibodies, suitable for experiments on various host species (rabbit, mouse, sheep, goat) and validated in various applications such as ELISA, Western blot, flow cytometry, immunohistochemistry and immunocytochemistry.

Read the application note