Detection of Aspergillus Fumigatus from Coriolis air samples by QPCR - Bertin Technologies

Detection of Aspergillus Fumigatus from Coriolis air samples by QPCR

Sources: Univ. Hospital of Besançon, and CNRS, Besançon, France

Context

Fungal infections represent a constant threat in hospitals, especially for high-risk patients hospitalized in hematology and bone marrow transplant units. Traditional monitoring involves impacted culture media that are incubated for several days so that the fungal species may grow and be identified on the basis of macroscopic and microscopic criteria. These systems are limited by their link with culture techniques, involving a time-lapse to results of around 7 days and by a maximal sampling rate of 100 L/min.
This study was carried out to test the Coriolis combined with QPCR to assess its performance for detecting A. fumigatus DNA under 2 days in hematology units and in non-hematology hospital areas [1].

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