Context
The generation of antigen-specific antibodies involves the injection of an immunogenic antigen-adjuvant emulsion in animals at defined time intervals. Preparing high-quality emulsions is a critical step to successfully generate specific antibodies. The two-syringe technique is widely employed as the standard method for emulsion preparation. This method has three important limitations: 1) reproducibility, due to operator preparation’s variability; 2) cost and time-efficiency, with a long procedure that requires large quantities of costly reagents, and 3) repetitive strain injury due to repetitive motions of thumb and wrist.
The Precellys Emulsion kit, combined with the use of the Minilys personal homogenizer, has been developed to overcome these limitations and ensure consistent and reliable preparations of high-quality emulsions.
This application note presents the results obtained by researchers of Innate Pharma SA (A clinical-stage biotechnology company in immuno-oncology based in Marseille) in an immunization project using the Precellys Emulsion kit and the Minilys personal homogenizer. Following preparation and injection of the immunogenic antigen-adjuvant emulsion, the quality of the triggered immune response was monitored by titration of mouse antisera.
