Context
RNA extraction is a routine technique in many molecular biology laboratories. High quality RNA is a prerequisite to succeeding in a series of downstream analyses. Nonetheless, isolating RNA from biological samples can be a challenging procedure.
RNA is notoriously unstable and sensitive to temperature. High quality and high yield RNA can be obtained from any type of tissue sample thanks to an optimal extraction process that relies on 2 steps: tissue homogenization and RNA purification. Finding the appropriate protocols for each one of these steps, and combining them to obtain the best out of any tissue sample is a mandatory milestone for most laboratories.
With its wide range of tissue homogenizers, Bertin Instruments addresses the first challenge of tissue disruption by using a powerful 3D bead-beating technology under the Precellys® brand. Bertin Bioreagent has thus developed an RNA extraction kit to complete the sample prep workflow resulting in the Precellys Tissue RNA Extraction Kit, a solution for all of your genomic applications.
In this Application Note, we present the results obtained using the Precellys Tissue Total RNA Extraction Kit for the isolation and purification of RNA from mouse heart tissue, and how they compare to some of our competitors.
