Intravenous immunoglobulin (IVIG) is known for its anti-inflammatory effects and is commonly used to treat auto-immune and systemic inflammatory diseases. Basophils are a type of white blood cells that are responsible for inflammatory reactions during the immune response and produce inflammatory mediators such as histamine and serotonin. Recently, it has been reported that, the anti-inflammatory effects of IVIG are mediated through basophils.
The object of this application note was to evaluate the effects of IVIG on human basophil functions based on several inflammatory biomarkers, including histamine. The Bertin Bioreagent Histamine ELISA kit was then used to measure histamine levels in human basophils.