Mastering Tissue Dissociation – Episode 1: THE HEART
05 Aug 2024
The dissociation of mouse heart tissue into single cells is a crucial step in many biomedical investigations However, obtaining viable...
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Sample preparation is critical to master molecules extraction from biological samples. Bertin Health & Life Sciences offers efficient and flexible Precellys tissue homogenizers for grinding any samples prior to analysis.
In order to extract the molecules of interest (extraction of RNA, DNA, proteins, metabolites, etc.) from biological samples, sample preparation procedures generally include a tissue homogenization or cell disruption step.
The Precellys® homogenizers from Bertin Health & Life Sciences use bead-beating technology to homogenize biological samples, such as human, animal or plant tissues, or to lyse micro-organisms. In this way, the biological samples are ground via multi-directional movement (3D bead-beating), the speed of which can reach up to 10,000 rotations per minute, depending on the equipment. Bead-beating is the gold standard method for tissue homogenization compared to standard techniques such as: mortar & pestle, sonication, blender, high pressure homogenizer. It takes just a few dozen seconds to homogenize the samples, which are then ready for an analysis of the molecules of interest (nucleic acids, proteins, metabolites) using different analysis techniques: PCR, sequencing, Western Blot, liquid chromatography, etc. This represents a considerable time saving that simplifies sample preparation protocols.
The high-performance characteristics of the Bertin Health & Life Sciences homogenizers, together with a wide variety of lysing and grinding kits, offer excellent reproducibility and a great degree of flexibility in a broad range of research areas including microbiology, pharmacology, cancer, forensic medicine, the environment, and even agronomy.
The Precellys® range allows you to prepare samples in full safety and without cross contamination, for both routine applications and R&D projects.
• For infrequent use, the Minilys® homogenizer allows you to prepare up to three samples at the same time. Its flexibility (0.5, 2 and 7 ml tubes) and its small size make it ideal for laboratories needing conventional sample preparation.
• The Precellys® 24 homogenizer can accommodate up to 24 biological samples in 2 ml and 0.5 ml tubes simultaneously. This robust appliance has been designed for daily use in laboratories with biological safety level 3.
• For even greater versatility, the Precellys® Evolution can accommodate up to 96 samples at the same time with six different tube volumes (from 0.3 ml to 15 ml), and meets the safety standards of laboratories with biological safety level 3.Avoid degradation of your heat-sensitive samples during homogenization with Cryolys® Evolution, the sample cooling system compatible with Precellys®
05 Aug 2024
The dissociation of mouse heart tissue into single cells is a crucial step in many biomedical investigations However, obtaining viable...
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