Context
Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder characterized by the progressive formation of fluid-filled cysts in the kidneys, often leading to complications, including hypertension, kidney stones, urinary tract infections, and eventually kidney failure. Affecting approximately 12 million people globally, ADPKD currently has no cure, and strategies to prevent cyst formation remain elusive.
Recently, studies have identified the transcription factor Glis2 as an early effector of polycystin signaling, with its expression being upregulated in cystic kidneys (Zhang, Rehman, Tian et. al., Nature Comm 15 (3698), 2024). Glis2 is a critical component of the cilia-dependent cyst activation (CDCA) pathway and has emerged as a potential therapeutic target for ADPKD.
This application note highlights the use of the Precellys Evolution tissue homogenizer to prepare normal and cystic kidney tissues from various mouse models of ADPKD. By analyzing changes in protein levels post-homogenization it was demonstrated the effectiveness of the Precellys Evolution in fractionating high-quality protein samples, which are essential for studying protein localization and understanding disease progression in ADPKD.
The Precellys Evolution‘s capacity for consistent, high-throughput tissue homogenization is a critical tool for advancing research into ADPKD and exploring potential therapeutic avenues.
