For researchers studying autosomal dominant polycystic kidney disease (ADPKD), accessing high-quality protein samples is essential for uncovering disease mechanisms and exploring therapeutic targets. The challenge lies in developing protocols that provide consistent and reproducible results while preserving sample integrity.
The Precellys Evolution Touch tissue homogenizer has proven to be a reliable tool for addressing these needs. In a recent study conducted by Yale University, a new protocol was developed to fractionate proteins from normal and cystic kidney tissues, enabling a deeper analysis of pathways such as Glis2 signaling.
Why This Protocol Matters
ADPKD research often focuses on molecular mechanisms like the cilia-dependent cyst activation (CDCA) pathway. The new protocol offers:
- Efficient tissue homogenization: Ensures high-quality cytosolic and nuclear protein fractions.
- Reproducibility: Reduces variability across samples for reliable comparative analysis.
- Scalability: Processes multiple samples efficiently, supporting high-throughput studies.