[PRECELLYS] Viable Salmonella typhimurium bacteria from infected nematodes

The nematode Caenorhabditis elegans is a powerful model system for the study of host-pathogen interactions. This study looked at innate immune response of C.elegans to Salmonella Typhimurium. To test whether the altered resistance of lys-7 and abl-1(tyrosine kinase ABL-1) mutant nematodes to Salmonella Typhimurium was due to a lower bacterial load within infected animals, wild type (N2), lys-7 and abl-1 animals were exposed to S. Typhimurium L1019, and infectious burden quantified over time through viable counts[1].

[1] Marsh EK, van den Berg MCW, May RC (2011) A Two-Gene Balance Regulates Salmonella TyphimuriumTolerance in the Nematode Caenorhabditis elegans. PLoS ONE 6(3): e16839.doi:10.1371/journal.pone.0016839