Context
In the living reign, DNA serves as the blueprint for all cellular processes, dictating the traits and functions of cells. RNA, on the other hand, acts as a messenger molecule, facilitating the translation of DNA-encoded genetic information into functional proteins, and their expression regulation, necessary for various cellular activities. Both DNA and RNA are essential components of cells, working together to ensure proper growth, development, and maintenance of tissues in the living reign.
The investigation of nucleic acids has become indispensable due to their pivotal functions. However, the isolation of DNA and RNA presents challenges. Obtaining high yields and purity of nucleic acids can pose difficulty. Moreover, the single-stranded nature of RNA is prone to instability, and susceptible to temperature fluctuations. Consequently, RNA degradation frequently occurs in sample preparation processes, leading to diminished yield and purity, along with the potential contamination by DNA.
In that frame, homogenization plays a key role in the yield and quality of nucleic acid extraction.
In this White Paper, discover across 4 optimized protocols for RNA & DNA extraction with Precellys homogenizers, how RNA & DNA extraction can get performed in dog skin, human skin, mouse kidney & frozen tumors in a high-throughput way ensuring increased quality and yield with limited molecules degradation.
