[PRECELLYS] DNA extraction from the fungal organisms Coprinopsis cinerea and Talaromyces emersonii

DNA extraction is an essential tool in the molecular analysis of fungi. The architecture of the fungal cell wall renders many fungi resistant to standard DNA extraction procedures employed for yeast and bacteria. Fungal DNA extraction protocols tend to be time consuming and/or result in a poor yield of DNA. While commercial kits are available for the extraction of fungal DNA, they do require a pre step of grinding (with or without liquid N2) for the initial breaking up of mycelia. Grinding can be time consuming, taking up to 30 minutes per sample depending on the fungal species. For high throughput screening and analysis, a rapid DNA extraction method is required for fungal samples. This method was tested on two different classes of fungal species; an ascomycete (T. emersonii) and a basidiomycete (C. cinerea).