Context
During the last few decades, research work has been done to investigate the different drugs of abuse in different types of biological samples (urine, saliva, hair, etc). One of the advantages of hair sample is the noninvasive collection.
Our goal for this application note is to develop a sample preparation method for a drug panel in hair using a single operation in LUXON-MS/MS and the Precellys 24 Touch (Bertin Technologies, Montigny-les-Bretonneux, France).
A proper sample preparation protocol is critical for MS-based analysis workflows. Indeed, the quality and reproducibility of the drug extraction can strongly influence MS results. This requires choosing an optimal protocol for the sample preparation step. The 3D-beating technology is considered the gold standard for sample pulverization. For this reason, Bertin Technologies has chosen 3-dimensional bead-beating technology to power the Precellys 24 Touch homogenizer (Bertin Technologies, Montigny-les-Bretonneux, France).
LUXON-MS/MS offers specificity combined with an ultra-fast analysis for an unrivaled screening method. To develop this application, we focused on performing a quick and simple sample preparation. Twenty drugs are analyzed simultaneously with quantitative screening results obtained in less than 8 seconds per sample. Each drug has been screened based on the industry cut-offs required.
