[PRECELLYS] Efficient extraction of pyridine nucleotides from mouse muscle

Measurement of pyridine nucleotides (NMN, NAD+, NADH, NADP, NADPH) in biological samples is vitally important given their pivotal, multifaceted roles in intermediary metabolism. We devised robust sample processing and LC/MS/MS methods to accomplish this challenging task given the inherent instability of these metabolites. Our assay precision and accuracy were enabled by the use of a Precellys Evolution homogenizer and custom-synthesized heavy isotope-labeled internal standards. We used these methods to profile the pyridine nucleotide pool in mouse gastrocnemius.