[PRECELLYS] Enzyme assay from frozen abdominal krill tissue

The goal of this study was to investigate whether Antarctic krill (Fig.1) possesses a circadian clock and whether such a clock controls key physiological processes of krill. To this end, we investigated temporal mRNA expression levels of the canonical clock gene cry2 in individual krill that were maintained both under a light-dark cycle and constant darkness. In addition, we tested whether gene expression of metabolic key enzymes in these krill show daily or circadian oscillations, and to what extent transcriptional oscillations of these enzymes also persist at the protein-activity level [1].

[1] Teschke, M. et al., PLoS ONE doi:10.1371/journal.pone.0026090