[PRECELLYS] High-throughput lipid extraction for the analysis of human brain lipids

Traditional lipid extraction techniques are the bottleneck for modern shotgun lipidomic studies. To overcome this problem, protocol comparisons were made between the traditional Folch extraction (using chloroform and glass-glass homogenization) and a high-throughput method combining methyl-tert-butyl ether (MTBE) with mechanical homogenization (Precellys-24, Bertin Technologies) [1].

[1] Sarah K. Abbott et al. An Improved High-Throughput Lipid Extraction Method for the Analysis of Human Brain Lipids, Lipids ISSN 0024-4201 Volume 48 Number 3 Lipids (2013) 48:307-318 DOI 10.1007/s11745-013-3760-z