[PRECELLYS] DNA extraction from mycobacteria

Genomic and proteomic research on mycobacterial diseases requires high quality and quantity preparation of DNA or proteins. Effective lysis of mycobacterial cells for DNA or protein extraction is demanding due to the mycobacteria’s robust and waxy cell wall features. Also, many mycobacteria are slow growers, often resulting in small amounts of starting material from a culture. Commercially available kits are not usually applicable to research methods on mycobacterial genomics and proteomics [1] [2].

[1] Kaser, et al. 2009. Optimized method for preparation of DNA from pathogenic and environmental mycobacteria.Appl.Environ.Microbiol. 75, no. 2 (January): 414-418.[2] Kaser, et al. 2010. Optimized DNAPreparation from Mycobacteria. Cold Spring Harb Protoc 2010, no. 4 (April) :pdb.prot5408.doi:10.1101/pdb.prot5408.