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Context
Precellys®24 homogenizer has been tested to perform polysomes immunoprecipitation (IP) and translating RNA extraction from IP. In our system, polysomes of some specific cell types of the cerebellum are immunoprecipitated, by expressing a GFP tagged ribosomal protein in these specific cell types with CRE mediated recombination. As these cell types represent a few percent of the whole cerebellum, we should observe an enrichment of specific genes in the IP fraction compared to the input fraction (whole cerebellum lysate before IP).
