Context
In maximizing protein extraction efficiency from biological samples, the choice of disruption technique plays a critical role. This study aims to compare two distinct methods: the 3D bead beating using the Precellys Evolution, and the sonication approach. Both methods were tested on fresh frozen pig liver biopsies to assess their effectiveness in protein yield and quality. Precellys employs the bead beating technology, utilizing high-speed agitation to disrupt cells and tissues efficiently. With customizable parameters and a range of lysing kits, the Precellys Evolution*, offers precise control over sample homogenization, ensuring reproducibility across various research applications.
Given the importance of optimizing protein extraction protocols for downstream applications, this study seeks to determine whether the Precellys homogenizer offers significant advantages over the conventional sonication technique. By focusing on pig liver tissue, we aim to evaluate the potential of these methods to enhance protein recovery in a commonly used and challenging biological sample.
