Context
In research on ophthalmology, mice are commonly used as models for retinal degeneration. Proteins expression analysis is needed to evaluate therapeutic agent efficiency. Concentrated protein extracts are difficult to obtain because of the small size of tissues. The ratio between volume for extraction and size of tissue is crucial for a sufficient yield. Currently, we are extracting proteins with a motorized pestle mixer, taking time and variability in extraction yield. The goal was to prepare a large number of sample with high reproducibility using a faster and easier methods.
