Context
The advent of next-generation sequencing techniques (NGS) has revolutionized the landscape of biology, allowing scientists to deepen their understanding of many diseases such as cancer. NGS workflows generally begin with a sample preparation step, whose success is crucial to ensure reliable sequencing results. An efficient and uniform homogenization is needed to obtain an adequate quantity and quality of nucleic acids. Mechanical lysis, and more specifically bead-beating technology, is considered the gold standard for sample homogenization.
In this White Paper, we present best practices for NGS sample preparation steps with the Precellys homogenizers over six optimized protocols, suitable for food, tissue, and fecal samples.